The incubation of adenosine 5´-monophoophate(AMP) with the homogenates of the epidermis and epidermis, which were oblaiined from the axiIlary skin of osmidrasis (bromidrosis) patients, resulted in the formation of adenosine andinorganic phosphate (Pi) withort further degradation, as demonstrated by paper chromatography.
The conversion of MP to adenosine in the skin was catalyzed by 5´-nucleotidase and alkaline: photosphatase. It was found that 5´-nucleotidase was present borth in the spidermis and dermis, being more active in the latter, and that the enzyme was responsible for more than 80% of the total AMP hydrolyzing activity present in the skin hamogenatts. Aikaline phosphatase was shown to be present mainly in the dermiis, and its contribution to AWP hydrolysis was insignificant at, pH 7.4.
From these results, it is evident that AMP is converted to adenosine chiefly by 5´ -nucleotidase, which is. present in the epidermis and dermis.
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